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戴志晖教授课题组在ANALYST发表研究论文

发布时间:17-08-04 15:26:26 文章来源:1 浏览次数: [ 字号:


Controllable Mn-doped ZnO nanorods for direct assembly of a photoelectrochemical aptasensor
Li, J (Li, Jing)[ 1,2 ] ; Dai, ZH (Dai, Zhihui)[ 1 ]*(戴志晖); Li, HB (Li, Hongbo)[ 2 ]*

[ 1 ] Nanjing Normal Univ, Coll Chem & Mat Sci, Jiangsu Key Lab Biofunct Mat, Nanjing 210023, Jiangsu, Peoples R China
[ 2 ] Yancheng Inst Technol, Coll Chem & Chem Engn, Yancheng 224051, Peoples R China

ANALYST,201706,142(12),2177-2184


A label-free photoelectrochemical (PEC) aptasensor for K+ was first constructed by direct self-assembly of the K+ aptamer onto the electrodeposited Mn-doped ZnO nanorods. In the presence of K+, the conformation of G-quadruplex changes to a K+-stabilized conformation, which can efficiently prevent the quercetin electron donor from reaching the functionalized photoanode, thus proportionately affecting the quercetin-enhanced photocurrent response. Under the optimal experimental conditions, the fabricated biosensor exhibited a linear response in the K+ concentration range of 0.012 to 12.32 nmol L-1, with a detection limit of 4.0 pmol L-1, which was 3-5 orders of magnitude lower than that of most of the recently reported methods. The presence of other ions did not interfere with the detection of K+, and the results in serum samples agreed well with those obtained by ICP-MS. Using K+ as the detection model, this novel PEC aptasensor exhibited a good performance in terms of ultrasensitivity, selectivity and simplicity, and it was economical. As regards the electrode modification, this work provided a convenient direct self-assembly strategy that did not require the use of ionic polymers such as PDDA as a binder. Thus, this study paves the way for the immobilization of molecular probes for label-free PEC aptasensing.


文章链接:
http://pubs.rsc.org/en/Content/ArticleLanding/2017/AN/C7AN00446J#!divAbstract
 


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