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戴志晖教授课题组在ANALYTICAL CHEMISTRY发表研究论文

发布时间:17-08-04 15:18:16 文章来源:1 浏览次数: [ 字号:


Sequence and Structure Dual-Dependent Interaction between Small Molecules and DNA for the Detection of Residual Silver Ions in As Prepared Silver Nanomaterials
Wang, ZY (Wang, Zhaoyin); Zhao, J (Zhao, Jian); Li, ZJ (Li, Zijun); Bao, JC (Bao, Jianchun); Dai, ZH (Dai, Zhihui)[ 1 ]*(戴志晖)

[ 1 ] Nanjing Normal Univ, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Sch Chem & Mat Sci, Nanjing 210023, Jiangsu, Peoples R China
[ 2 ] Nanjing Normal Univ, Jiangsu Key Lab Biofunct Mat, Sch Chem & Mat Sci, Nanjing 210023, Jiangsu, Peoples R China

ANALYTICAL CHEMISTRY,201706,89(12)


Investigations on interaction between small molecules and DNA are the basis of designing advanced bioanalytical systems. We herein propose a novel interaction between heterocyclic aromatic compounds (HACs) and single-stranded DNA (ssDNA). Taking methylene blue (MB) as a typical HAC, it is found that MB can interact with cytosine (C)-rich ssDNA in an enthalpy-driven process. The interaction between MB and C-rich ssDNA is sequence and structure dual-dependent: at least three consecutive C and single-stranded structure are necessary, affecting the fluorescence response of metal nanoparticles. With the exception of the single-stranded structure, double stranded, i-motif; and C-Ag-C mismatch structures will remarkably impede the interaction with MB. UV vis absorption, fluorescent, and electrochemical curves demonstrate that the conjugated system, electron transition, and electron transfer of MB are remarkably affected by MB-C-rich ssDNA interaction. In particular, the absorption peak of MB at 664 nm decreases, and a new peak at 538 nm emerges. Therefore, the interaction can be characterized by a colorimetric and ratiometric signal. Relying on the inhibition of C-Ag-C mismatch and the enhanced analytical performances of the ratiometic signal, the MB-C-rich ssDNA interaction is further employed to quantify silver ions (Ag+) selectively and sensitively. In addition, since silver nanomaterials cannot introduce C-Ag-C mismatch, the fabricated biosensor is able to sense residual Ag+ in silver nanoparticles and silver nanowires, which is of great value in the precise and economical preparation of silver nanomaterials.


文章链接:
http://pubs.acs.org/doi/abs/10.1021/acs.analchem.7b01238
 


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