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周宁琳教授课题组在RSC ADVANCES发表研究论文

发布时间:17-04-10 10:39:05 文章来源:化科院 浏览次数: [ 字号:


pH-Sensitive N-doped carbon dots-heparin and doxorubicin drug delivery system: preparation and anticancer research
Zhang, M (Zhang, Ming)[ 1,2 ] ; Yuan, P (Yuan, Ping)[ 1,2 ] ; Zhou, NL (Zhou, Ninglin)[ 1,2,3 ]*(周宁琳); Su, YT (Su, Yutian)[ 1,2 ] ; Shao, MN (Shao, Maoni)[ 1,2 ] ; Chi, C (Chi, Cheng)[ 1,2 ]

[ 1 ] Nanjing Normal Univ, Coll Chem & Mat Sci, Jiangsu Collaborat Innovat Ctr Biol Funct Mat, Nanjing 210023, Jiangsu, Peoples R China
[ 2 ] Jiangsu Engn Res Ctr Biomed Funct Mat, Jiangsu Key Lab Biofunct Mat, Nanjing 210023, Jiangsu, Peoples R China
[ 3 ] Nanjing Zhou Ninglin Adv Mat Technol Co Ltd, Nanjing 211505, Jiangsu, Peoples R China

RSC ADVANCES,201701,7(15),9347-9356

In this study, doxorubicin (DOX) hydrochloride as a model drug, N-doped carbon dots as a drug carrier, and heparin as an auxiliary medicine were selected to design and prepare a multi-functional drug delivery system with pH-triggered drug release. The CDs were anchored onto heparin via chemical bonds and DOX was then loaded on CDs-Hep by taking advantage of the electrostatic interactions between DOX and CDs-Hep. The structures of all the intermediates and final products were characterized and confirmed by H-1 NMR and FT-IR spectroscopies. The CDs-Hep/DOX drug delivery system exhibited good stability. However, in acidic buffer, Hep and DOX release rate was accelerated and it was pH-responsive. In vitro and in vivo studies confirmed the high biocompatibility and low-toxicity of the CDs. An MTT assay showed that inhibition rate of CDs-Hep/DOX for HeLa, MCF-7 and A549 cells was close to that of DOX, indicating that the prepared drug system has a higher toxicity for tumor cells and can achieve an effective therapeutic effect. This systemic evaluation suggests that the introduction of Hep improves blood compatibility. In addition, the internalization of CDs-Hep/DOX by A549 cells was further confirmed using laser scanning confocal microscopy. As a result, a therapy was achieved due to the incorporation of Hep and DOX.


文章链接:
http://pubs.rsc.org/en/Content/ArticleLanding/2017/RA/C6RA28345D#!divAbstract
 


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