Highly efficient enrichment ofN-glycopeptides by two-dimensional Hf-based metal-organic framework nanosheets
Yang, SS (Yang, Shi-Shu)[ 1 ] ; Wang, C (Wang, Chen)[ 1 ] ; Xiao, J (Xiao, Jing)[ 1 ] ; Yu, XZ (Yu, Xizhong)[ 2 ] ; Shang, WB (Shang, Wenbin)[ 2 ] ; Chen, DD (Chen, David Da Yong)[ 1,3 ]*; Gu, ZY (Gu, Zhi-Yuan)[ 1 ]*（古志远）
[ 1 ] Nanjing Normal Univ, Coll Chem & Mat Sci, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Jiangsu Key Lab Biofunct Mat, Nanjing 210023, Peoples R China
[ 2 ] Nanjing Univ Chinese Med, Coll Clin Med 1, Key Lab Metab Dis Chinese Med, Nanjing 210023, Peoples R China
[ 3 ] Univ British Columbia, Dept Chem, Vancouver, BC V6T 1Z1, Canada
Highly efficient enrichment of target glycopeptides plays a crucial role in glycoproteome research. Owing to their large surface area and excellent hydrophilicity, 2-D Hf-BTB nanosheets showed effective and selective enrichment of 78 glycopeptides derived from 29 glycoproteins with 90N-glycosylation sites from just 2 mu L of human serum.