Zwitterionic Polymer-Grafted Polylactic Acid Vascular Patches Based on a Decellularized Scaffold for Tissue Engineering
Zhang, J (Zhang, Jun)[ 1 ] ; He, L (He, Lei)[ 1 ] ; Wei, G (Wei, Guo)[ 1 ] ; Jiang, XF (Jiang, Xuefeng)[ 1 ] ; Fu, L (Fu, Lei)[ 1 ] ; Zhao, Y (Zhao, Yue)[ 1 ] ; Zhang, LX (Zhang, Luxia)[ 1 ] ; Yang, LT (Yang, Lutao)[ 1 ] ; Li, YJ (Li, Yajuan)[ 1 ] ; Wang, YT (Wang, Yutong)[ 2 ] ; Mo, H (Mo, Hong)[ 1 ]*（莫宏）; Shen, J (Shen, Jian)[ 1 ]*（沈健）
[ 1 ] Nanjing Normal Univ, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Natl & Local Joint Engn Res Ctr Biomed Funct Mat, Sch Chem & Mat Sci,Jiangsu Engn Res Ctr Biomed Fu, Wenyuan Rd 1, Nanjing 210023, Jiangsu, Peoples R China
[ 2 ] Nanjing Forestry Univ, Coll Mat Sci & Engn, Longpan Rd 159, Nanjing 210037, Jiangsu, Peoples R China
ACS BIOMATERIALS SCIENCE & ENGINEERING，201909,5(9),4366-4375
More than 10 million people suffer from cardiovascular diseases, and diseased blood vessels need to be treated with vascular patches. For a vascular patch, good affinity for endothelial progenitor cells is a key factor in promoting the formation of endothelial tissue-endothelialization. To construct such a vascular patch with good cell affnity, in this work, we first synthesized a reactive zwitterionic organophosphate containing a phosphorylcholine headgroup: 6-(acryloyloxy)hexyl-2-(N-isopropyl-N,N-dimethylammonio)ethyl phosphate (AHEP). We then grafted AHEP onto a polylactic acid (PLA)-coated decellularized scaffold to obtain a vascular patch. Its hydrophilicity and biocompatibility were investigated. Its in vivo performance was also examined in a pig model with B-ultrasonography, Doppler spectra, and computed tomography angiography. The vascular patch demonstrated a nonhemolytic property, noncytotoxicity, long in vitro coagulation times, the strong ability to resist platelet adhesion, and a good affinity for endothelial progenitor cells. The vascular patch was able to maintain the long-term patency (5 months) of surgical arteries. Hence, the zwitterionic polymer-grafted PLA vascular patch may be a promising candidate for vascular tissue engineering.