A detection method in living plant cells for rapidly monitoring the response of plants to exogenous lanthanum
Cheng, MZ (Cheng, Mengzhu)[ 1 ] ; Wang, LH (Wang, Lihong)[ 2 ] ; Yang, Q (Yang, Qing)[ 1 ] ; Huang, XH (Huang, Xiaohua)[ 1 ]（黄晓华）*
[ 1 ] Nanjing Normal Univ, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Jiangsu Key Lab Biomed Mat, Sch Chem & Mat Sci, Nanjing 210023, Jiangsu, Peoples R China
[ 2 ] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY，201808,158,94-99
The pollution of rare earth elements (REEs) in ecosystem is becoming more and more serious, so it is urgent to establish methods for monitoring the pollution of REEs. Monitoring environmental pollution via the response of plants to pollutants has become the most stable and accurate method compared with traditional methods, but scientists still need to find the primary response of plants to pollutants to improve the sensitivity and speed of this method. Based on the facts that the initiation of endocytosis is the primary cellular response of the plant leaf cells to REEs and the detection of endocytosis is complex and expensive, we constructed a detection method in living plant cells for rapidly monitoring the response of plants to exogenous lanthanum [La(III), a representative of REEs] by designing a new immuno-electrochemical method for detecting the content change in extracellular vitronectin-like protein (VN) that are closely related to endocytosis. Results showed that when 30 WA La(III) initiated a small amount of endocytosis, the content of extracellular VN increased by 5.46 times, but the structure and function of plasma membrane were not interfered by La(III); when 80 mu M La(III) strongly initiated a large amount of endocytosis, the content of extracellular VN increased by 119 times, meanwhile, the structure and function of plasma membrane were damaged. In summary, the detection method can reflect the response of plants to La(III) via detecting the content change in extracellular VN, which provides an effective and convenient way to monitor the response of plants to exogenous REEs.